Facts-enriched characterization of cell populations. Morphological information from pictures can incorporate on the richness of flow cytometry data. As an example, the figure shows an normally common apoptosis assay employing Annexin V and PI, introducing cell imaging to characterize cells in Each individual inhabitants to expose morphologically unique options. These insights could not happen to be received from multiplex staining by itself.

Whether you’re a newbie or an authority, investigate our means to progress your analysis and expertise in flow cytometry.

(B) Uncooked 264.7 macrophages are sometimes annotated inaccurately with respect to particle rely because of their complexity and noncircular borders. The number of singlets available for Examination raises after the user trains and employs a more exact design.

Uncover new insights into your experimental information by visualizing populations making use of blended fluorescence and morphology facts. Our software package interface wizards permit downsampling, concatenation (merged files) and dimensionality equipment which are constructed into your application for an intuitive, streamlined information Evaluation practical experience.

*Pricing is indicative only which is subject to alter. You should Get hold of your Agilent consultant with any inquiries. The knowledge established forth herein isn't a proposal or a motivation, equally of which happen to be matter to credit score approval, stipulations, and availability with Agilent. Agilent Economic Methods is on the market in picked nations.

Here, a qualified person has gated singlets confidently. Right after evaluating the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate has over 4% aggregates.

Pair your Attune flow cytometer by having an autosampler for elevated workflow effectiveness, and add robotic integration for optimum throughput.

Reliable graphic top quality even at higher throughput Acoustic concentrating and a click here large-pace digital camera Mix to impression these T cells persistently at reduced or superior flow premiums. Very easily change aim and digital camera options to satisfy experimental prerequisites.

Early indications of this contamination experienced to begin with been dismissed as debris. Backgating to find out new populations. Backgating imaged cells to the Attune CytPix Flow Cytometer also allows you to use morphological features to discover interesting subpopulations that will not be evident from flow cytometry details on your own.

Possibly most of all, these gatherings consist of cells of Evidently distinctive phenotypes which could lead on to incorrect conclusions pertaining to double good occasions (specifically in unusual populations).

Seem waves empower exact cell alignment, leading to Fantastic knowledge high-quality and ~10X more quickly sample flow premiums.

Software note: Automatic impression Examination cuts down person-to-consumer variability in flow cytometry gating tactics

Leverage the strength of Superior technological innovation and innovation to speed up your discoveries. Trust inside the Attune Flow Cytometer family to deliver the dependability, pace, and precision that set your work aside.

Flexible payment plan from Agilent Economical Methods enable you to have the crucial instrumentation you may need for quick undertaking, or to expand your lab.

At the guts of every Attune Flow Cytometer lies our revolutionary acoustic concentrating engineering, which makes use of sound waves to meticulously align cells inside the flow cell. This impressive approach surpasses traditional hydrodynamic procedures, causing faster processing speeds, exceptional information excellent, and greater sample throughput.

The Attune Xenith cytometer is a substantial-parameter spectral analyzer, supplying equally spectral unmixing and standard flow abilities for comprehensive and functional cell analysis.

Agilent Worth Assure ensures you a minimum of ten years of instrument use in the day you buy. Otherwise, we will credit history you Together with the residual worth of the process towards an upgraded design.

What gave the impression to be a homogeneous inhabitants is definitely a lot more numerous—an Perception that needs to be considered when interpreting benefits.